Fig. 1

A high expression level of the lncRNA NEAT1 in human ovarian cancer cells suppressed miR-214-3p expression and promoted cell proliferation. A-B Human ovarian cancer tissues had more expression of NEAT1 in comparison to the paired non-tumor adjacent normal tissues. A Representative images of in situ RNA hybridization using ovarian cancer tissues slide and normal slides. B The positive rate of NEAT1-expression cells was summarized. n = 3 for each group. C The relative RNA levels of miR-214-3p and NEAT1 in human ovarian surface epithelial cell line IOSE80 and human ovarian cancer cell lines SKOV-3 and A2780 were determined by qPCR. D The luciferase activity of the reporter vectors was detected in SKOV-3 cells at 48 h after co-transfection of the plasmid expressing wild type or mutant NEAT1 alone (without miRNA), or together with the negative control (NC) mimic, or the miR-214-3p mimic. n = 5 for each group. E–F The relative levels of NEAT1 (E) and miR-214-3p (F) in Blank SKOV-3 cells, stable SKOV-3 cells transfected with empty vector or NEAT1-expressing vector, SKOV-3 cells infected with shLncRNA NEAT1- or shScramble-expressing lentivirus, and stable NEAT1-expressing SKOV-3 cells with additional transfection of miR-214-3p mimic were determined by qPCR. G-H The proliferation of blank SKOV-3 cells (G) and A2780 cells (H) or these cells transfected with empty vector or NEAT1-expressing vector (mLncRNA NEAT1), or infected with shLncRNA NEAT1- or shScramble-expressing lentivirus was determined by CCK-8 assays. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, between the indicated groups. Each group was tested with three samples