Fig. 2

NEAT1 expression in human ovarian cancer cells inhibited apoptosis and promoted cell migration and invasion. A-D Apoptosis rates in untreated SKOV-3 or A2780 cells (blank), stable NEAT1-expression cells (mlncRNANEAT1) or control vector-transfected cells (vector), and NEAT1-knockdown cells with infection of shRNA-expressing lentivirus (shlncRNANEAT1) or the corresponding control cells with infection of scramble shRNA-expressing lentivirus (shScramble) were measured by Annexin-V/7-AAD flow cytometrical staining. Representative flow profiles for SKOV-3 (A) and A2780 (B) cells after culturing for 24 h, 48 h and 72 h are shown. Starting from the bottom left (Q3) and moving in a counterclockwise direction, the quadrants represent live cells (Q3), early apoptotic cells (Q4), late apoptotic cells (Q2), and necrotic cells (Q1), respectively. The percentages of apoptotic cells (Annexin V-positive cells among total cells; C for SKOV-3 cells and D for A2780 cells) were summarized. E-H The abilities of migration (E, G) and invasion (F, H) of SKOV-3 (E, F) or A2780 cells (G, H) were determined by transwell assays. I-L Qualification of migration (I, K) and invasion (J, L) of SKOV-3 (I, J) or A2780 (K, L) cells. Representative images are shown, and the numbers of migrated or invaded cells were summarized. n = 3 for each group; *P < 0.05, **P < 0.01, ***P < 0.001, between the indicated groups