Fig. 4

NEAT1 promoted angiogenesis in xenografted human ovarian cancer tissues in nude mice. A-G Nude mice were inoculated with untreated SKOV-3 or A2780 cells (blank), stable NEAT1-expression cells (mlncRNANEAT1) or control vector-transfected cells (vector), NEAT1-knockdown cells with infection of shRNA-expressing lentivirus (shlncRNANEAT1) or the corresponding control cells with infection of scramble shRNA-expressing lentivirus (shScramble). Tumor volume was monitored every 7 days starting 17 days after tumor cell inoculation, and mice were euthanized at 30 days after inoculation. A-B Tumor growth curves of SKOV-3 (A) or A2780 (B) cells are shown. C Representative HE staining images show the angiogenesis in tumor tissues. Scale bar, 50 µm. D-E The protein levels of angiogenesis-related molecules in tumor tissues derived from SKOV-3 (D, F) or A2780 (E, G) cells were determined by western blot assays. The representative bands images are shown (D-E), and relative protein levels were summarized (F-G). n = 5 for each group; *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant, between the indicated groups