Fig. 1

Adipose conditioned media (ACM) downregulate CBX7 and enhance migratory capacity in ovarian cancer cells. A panel of human ovarian cancer cells were cultured for 5 days in ACM from omental explants isolated from a female patient with a benign gynecological condition. No treatment control cells (NT) were cultured in growth media. A Levels of CBX7 were determined by Western blot analysis; B Representative microscopic images show acquisition of a fibroblastic spindle-like morphology; C Western blot analysis for EMT markers in OCSC1-F2 human ovarian cancer cells; D top panel, OCSC1-F2 human ovarian cancer cells were cultured in ACM or growth media for 5 days, trypsinized, washed, and placed in trans-well inserts with growth media. Note enhanced migration in cells previously cultured with ACM; bottom panel, quantification of migration (n = 8); *** p < 0.0001. Data are presented as mean ± SEM. Unpaired t-test was used to determine statistical significance