Fig. 2

Characterization of exosomes derived from adipose conditioned media (ACM). Exosomes were isolated from ACM by differential ultracentrifugation. A Representative particle size distribution (ave = 137 nm) of ACM-derived exosomes analyzed by Nanoparticle Tracking Analysis; B Representative transmission electron microscopy image showing the doughnut-shaped morphology of the isolated exosomes; C Representative Western blot analysis of ACM-derived exosomes showing expression of exosomal markers CD63 and CD9 and absence of the endoplasmic reticulum marker, calnexin; Protein lysate of Adipose-Derived Mesenchymal Stem Cells (ADMSC) is used as positive control of calnexin. D Representative images from ExoView analysis showing majority of captured exosomes are positive for the adipocyte marker, CD36, and negative for the macrophage marker, CD11b; E Quantification of D and showing data from 6 individual patients. Data are presented as mean ± SEM (n = 6). Unpaired t-test was used to determine statistical significance. Data shown are for exosomes 654 and 652. Similar results were observed with other exosomal preparations