Fig. 3
Adipose-derived exosomes are internalized by human ovarian cancer cells via endocytosis and induce down-regulation of CBX7. A Labelled ACM-derived exosomes were internalized by mCherry-labelled OCSC1-F2 cells; B Human R182 ovarian cancer cells were treated for 48 h with adipose-derived exosomes (5 × 108 exosomes/ml) isolated from patients in Table 1. top panel, levels of CBX7 determined by Western blot analysis. * denotes preparations able to down-regulate CBX7. bottom panel, densitometry quantification of top panel; C OCSC1-F2 human ovarian cancer cells were treated with ACM, exosomes (exo), exosome-depleted ACM (ACM-exo) or exosome with Nystatin and levels of CBX7 determined by Western blot analysis; (D) OVCAR3 human ovarian cancer cells were treated with ACM, exosomes (exo), or exosome-depleted ACM (ACM-exo) and levels of CBX7 determined by Western blot analysis