Fig. 5

Cytokine/chemokine concentrations in ascites reflect BRCA-dependent differences in response to treatment. Ascites fluids were collected at humane endpoint (n = 4 per group). Cytokines were quantified using the LEGENDplex Mouse Cytokine Release Syndrome flow-based assay. A Heatmap showing the normalized concentrations of cytokines in the ID8 Trp53^−/− Brca1^−/− and ID8 Trp53^−/− Brca2^−/− models. Each box represents the mean of 4 replicates. B-G Histograms indicating the concentrations that are significantly different from isotype control or the combination in at least one model. The histograms are divided based on tumour genotype: (B-D) shows data from the ID8 Trp53^−/− Brca1^−/− ascites and (E–G) the Trp53^−/− Brca2.^−/− ascites fluid. Each dot represents the ascites supernatant from one biological replicate. Mean values with SD are shown. Analysis was done using a one-way ANOVA with Tukey’s multiple comparison; *p < 0.05, **p < 0.01, ***p < 0.001