Fig. 6

Deficiencies in Brca1 and Brca2 differently influence the expression of immune-related factors in the ovarian tumour microenvironment. A Heatmap showing the relative expression of immune-related genes in intraperitoneal tumours. Each box represents the mean value of log-normalized bulk RNA-seq data from 4–6 biological replicates. Gene names were replaced by the corresponding protein name. B Pathway analyses for three gene sets that demonstrate the activities of the NF-kB, angiogenic and PD-L1 pathways. The activity of the pathway was approximated by measuring the expression of 12–53 genes which are known to be downstream for the activity of each protein of interest. C Immunohistochemical staining images (left) and quantification of CD31⁺ cells (brown) in Brca-deficient tumours collected at the humane endpoint (n = 4) from the mice in the isotype control group. Quantifications are based on the percentage area of CD31⁺ cells in relation to all nucleated cells. D Histograms presenting the concentrations of VEGF in the ascites fluid collected from mice harbouring Brca1- and Brca2-deficient tumours. Cytokines were quantified using the LEGENDplex Mouse Cytokine Release Syndrome flow-based assay. E Histograms presenting the percentage of PD-L1⁺ cells in untreated ID8 Trp53^−/− Brca1^−/− and ID8 Trp53^−/− Brca2.^−/− cell lines. Expression levels were quantified using flow cytometry. Mean ± SEM are shown for all histograms. Analysis was done using the student t test; *p < 0.05, ***p < 0.001