Fig. 4

Validation of sh-UBE2S gene transfection efficiency in cisplatin-resistant cell lines. A Twenty-four hours after lentiviral transfection of Sh-UBE2S, the transfection efficiency was observed by fluorescence microscopy. Green fluorescence indicates successful transfection. B PCR-validated histogram of changes in gene expression levels following lentiviral transfection of Sh-UBE2S. Statistical analysis was performed using 2-∆∆Ct values. RT-qPCR was used to detect the transfection efficiency of UBE2S. Data are presented as mean ± standard deviation (SD). C Western blotting experiments verifying the changes in gene protein levels after lentiviral transfection of Sh-UBE2S and histograms of the results of statistical analysis (*p < 0.05, **p < 0.01, ***p < 0.001)