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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: In-vitro generation of follicle-like structures from human germ cell-like cells derived from theca stem cell combined with ovarian somatic cells

Fig. 3

Immunofluorescence staining of DAZL and GDF9 for negative and positive controls. Mouse GV (A) and MII oocytes (B) as a positive control and hTSCs as a negative control (C). a. Phase-contrast image of mouse oocytes and hTSCs. b. Mouse oocytes and hTSCs after staining the nuclei with DAPI. c. Immunofluorescence staining of GDF9 and DAZL. d. Merged DAPI and primary antibody-secondary antibody-FITC staining of GDF9 and DAZL. Scale bars (A): 50 μm, (B): 100 μm and (C): 10 μm

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Journal of Ovarian Research

ISSN: 1757-2215

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