Fig. 7

The effects of ASC and ASC-CM on the estrus cyclicity, after 4 weeks of treatment. A Stained slides of vaginal smears in the normal rats. a In the proestrus phase, the smears mainly contained epithelial cells (EC). b In the estrus phase, the smears contained cornified cells (CC). (c) In the metestrus phase, the smears contained CC, EC and leucocyte cells (LC). d In the diestrus phase, the smears mainly contained LC. (hematoxylin staining; magnification, × 40) (B) The scatterplots represent the proportion of time spent in each estrus cycle in the four groups of animals. The Y axis represents the percentile range from 0–100. * shows significant Differences vs Sham group (*P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001). Data are expressed as mean ± SEM. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test. Abbreviations: CTRL, Control group; ASC, Adipose stem cell group; ASC-CM, Adipose stem cell-conditioned media group