Table 1 List of specific primers used in real-time polymerase chain reaction assay
Gene name | Accession no | Designed oligonucleotide (5’ → 3’) | Product size (bp) |
|---|---|---|---|
DNMT1 | NM_053354.3 | F: CAAGATGCCAGCACGAACAG R:AGCCATCTCTTTCCAAGTCTTTG | 110 |
DNMT3A | XM_039112666.1 | F:CGGATAATACCTTCTCTGAAGCCC R: CCTGTTCCTCTCCTTCCTTTCG | 162 |
DNMT3B | NM_001396349.1 | F:AGAAGAGGGTGCTAGTGGGTATG R: TTCATCTCCATCATCCGCTTCAC | 250 |
HDAC1 | NM_001025409.1 | F: CCAGAAGCCAAAGGGGTCAAA R: TGTGCGCTGGTCCCTATCTAG | 182 |
HDAC2 | NM_053447.1 | F: GAGGCGGCAAGAAGAAAGTGT R: GTCATCCGGATCCTATGGGG | 100 |
ESRα | NM_012689.1 | F: GAGCACATTCCTTCCTTCCGT R: AGGCTTTGGTGTGAAGGGTC | 191 |
ESRβ | NM_012754.3 | F: CGTTCTGGACAGGGATGAGG R: GCAGAAGCCAAGGGGTACAT | 168 |
β-actin | NM_031144.3 | F: CCCGCGAGTACAACCTTCTT R: CGCAGCGATATCGTCATCCA | 83 |