Fig. 3
From: Bisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stress
Reactive oxygen species (ROS) levels were detected after high concentrations of BPA (12 h treatment of 200 μM). Fluorescence microscopy (A) and flow cytometry (B) for the detection of ROS in mpGCs after BPA exposure. C shows the results of the statistical figure (B). Both fluorescence observation and flow cytometry showed that mpGCs produced a large number of ROS after high concentration and high BPA treatment; (D) Western blot results showed that the expression level of γ-H2AX in the high concentration treatment group was obviously higher than that in the control group. ***, P < 0.001