Fig. 1

Assembly of VLP displaying human or mouse MUC16 20mer epitopes. (A). HEK293 cells were transfected with expression constructs for wild type (wt) HPV16 L1 and L2 (L1-L2) or HPV16 L1 with 20 aa inserts of the RG1 epitope (L1-RG1), mMUC16 (L1-mMUC16) or hMUC16 (L1-hMUC16). Three days later, cell lysates were harvested and subjected to Western blot analysis with L1-specific monoclonal antibody, CamVir-1. Lysates of HEK293 cells transduced with L1-mMUC16 or L1-hMUC16 expression vectors were fractionated on an Optiprep step gradient, and VLP-containing fractions analyzed by SDS-PAGE and Coomassie staining. C and D). Transmission electron microscopy of fraction 1 from B for L1-mMUC16 (C) or L1-hMUC16 (D)