Fig. 2

ELISA of antibody response to vaccination with DNA vectors expressing L1 with 20aa inserts of the RG1 epitope, mMUC16 or hMUC16. Female mice (n = 10/group) were vaccinated 3 times i.m. followed by electroporation with 30 µg DNA in PBS, and serum was harvested 2 weeks later (A-D). Sera were analyzed by ELISA against mMUC16 (A), hMUC16 (B) or RG1 (D) 20mer N-terminally biotinylated peptide on streptavidin coated plates, or HPV16 L1-VLP coated plates (C)