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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Virus-like particle vaccine displaying an external, membrane adjacent MUC16 epitope elicits ovarian cancer-reactive antibodies

Fig. 3

Malemide coupling chemistry to covalent link MUC16 peptide to surface sulfhydryl on L1 VLP. Purified HPV16 L1-VLP (A) or L1-RG1-VLP (B) were reduced and coupled with malemide-linked mMUC16 or hMUC16 peptide. Samples and molecular weight standards (right of panel) were separated by SDS-PAGE and the gels stained with Coomassie (A, B), or the samples were viewed by transmission electron microscopy (C-H).

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