Fig. 4

ELISA of antibody response to vaccination with VLP either mixed with or coupled to 20aa mMUC16 or hMUC16 peptides. Female mice (n = 10/group) were vaccinated 3 times i.m. with alum-formulated L1 VLP or L1-RG1-VLP VLP either mixed (mix) with or coupled (~) to 20aa mMUC16 or hMUC16 peptides, and serum was harvested 2 weeks later (A-D). Sera were analyzed by ELISA against mMUC16 (A), hMUC16 (B) or RG1 (D) 20mer N-terminally biotinylated peptide on streptavidin coated plates, or HPV16 L1 VLP coated plates (C)