Fig. 5

Binding of MUC16-specific antibody to CA125 + ovarian cancer cell surface and lysates. A) Monoclonal antibody VK8 to CA125 and serum of a mouse vaccinated with hMUC16 peptide coupled to L1-RG1-VLP was used in an immunoblot to probe cell lysate of OVCAR3 (positive control) or SKOV3 (negative control). B and C). Monoclonal antibody VK8 or mouse antisera described in Figs. 2 and 4 were bound with the surface of intact OVCAR3 (B) or SKOV3 cells (C). After washing, bound mouse antibody was detected using PE-labeled anti-mouse IgG and flow cytometry. Mean fluorescent intensity (MFI) was determined