Fig. 5
From: MiR-134-3p targets HMOX1 to inhibit ferroptosis in granulosa cells of sheep follicles
oar-miR-134-3p regulated HMOX1 expression. A The mRNA expression of oar-miR-134-3p determined by RT‒qPCR in GCs transfected with oar-miR-134-3p mimics, inhibitor, and NC. B The mRNA expression of HMOX1 determined by RT‒qPCR in GCs transfected with oar-miR-134-3p mimics, inhibitor, and NC. C Relative expression of HMOX1 detected by Western blot in GCs transfected with oar-miR-134-3p mimics, inhibitor, and NC. Original blots are presented in Supplementary Fig. 3. D Luciferase activities in cells transfected with a luciferase plasmid containing the wild-type (WT) or mutated (MUT) HMOX1 3′ UTR and either the oar-miR-134-3p mimic or a negative control (NC). *P < 0.05, **P < 0.01, ***P < 0.001