Fig. 2

High TET1 and GSN expression significantly correlate with increased recurrence and shortened survival of OVCA patients. (A) OVCA public data sets (Kaplan-Meier Plotter) was analyzed for TET1 correlation with patient survival and (B) GEPIA was used to demonstrate the correlation between GSN and TET1 gene expression in ovarian cancer (OVCA), cholangiocarcinoma (CHOL), cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), and rectum adenocarcinoma (READ) tissues. Significantly higher pGSN mRNA and protein contents observed in OVCA resistant cells (endometrioid, A2780cp; HGS, TOV3133R) compared to sensitive cells (endometrioid, A2780s; HGS, TOV3133G) which is associated with decreased chemoresponsiveness. OVCA cells were treated with or without CDDP (10 µM; 24 h). (C) Apoptosis was morphologically determined by Hoechst 33,258 nuclear staining and cell viability examined using CCK8 assay (D) pGSN and GAPDH (loading control) protein contents were assessed by Western blotting (E) pGSN mRNA content relative to ACTB (loading control) was assessed by qPCR. (mean ± SEM; n = 3). Differences between all the groups were evaluated using two-way and three-way ANOVA followed by Tukey’s multiple comparison test; p*<0.05, p**<0.01, p***<0.001, p****<0.0001