Fig. 3

TET1 is differentially expressed between chemosensitive and chemoresistant OVCA cells, however, no significant differences in the TETs activity observed different OVCA cell types. OVCA cells were treated with or without CDDP (10 µM; 24 h). (A) TET1 mRNA content relative to ACTB (loading control) was assessed by qPCR, (B) TET1 and GAPDH (loading control) protein contents were assessed by Western blotting, and (C) TET activity was assessed using epigenase 5mC-hydroxylase TET activity assay kit (mean ± SEM; n = 3). Differences between all the groups were evaluated using two-way ANOVA followed by Tukey’s multiple comparison test; p*<0.05, p**<0.01, p***<0.001, p****<0.0001