Fig. 4

DNA methylation plays a role in the regulation of pGSN expression in OVCA cells. A2780cp and A2780s cells were treated with (A) BobCat 339; TETi (0-100 µM; 48 h) and (B) 5-aza; DNMTi (0–10 µM; 48 h), respectively, followed by CDDP treatment (10 µM; 24 h). pGSN and GAPDH (loading control) protein contents were measured by Western blotting and apoptosis was morphologically determined by Hoechst 33,258 nuclear staining (mean ± SEM; n = 3). (p*<0.05, p**<0.01, p***<0.001, p****<0.0001 versus the corresponding control group: BobCat 339/5-aza; 0 µM)