Fig. 5

DNA methylation alters pGSN methylation in OVCA cells. Chemoresistant (A2780cp, TOV3133R) and chemosensitive (A2780s, TOV3133G) OVCA cells of were treated with BobCat 339; TETi (100 µM; 48 h) and 5-aza; DNMTi (5 µM; 48 h), respectively. DNA methylation percentage was quantitatively assessed by EpiTYPER DNA methylation technology. Methylation % differences (A) at multiple CpG sites between endometrioid sensitive (A2780s) vs. resistant (A2780cp) cells, (B) at multiple CpG sites in endometrioid resistant cells after TETi treatment (C) between HGS sensitive (TOV3133G) and resistant (TOV3133R) cells (D) at multiple CpG sites in HGS resistant cells after TETi treatment (E) schematic diagram of methylation status (F) schematic diagram of GSN gene. The region of interest for pGSN was analyzed in 2 fragments. Circles mark the position of CpG sites and color indicates the methylation status of CpG site. The color scale in each top left corner indicates the methylation level. Circle color from red to yellow indicates the methylation ranging from 0-100%. (mean ± SEM; n = 3)