Fig. 6

TET1 appears to have an inverse relationship with pGSN expression. (A) A2780cp cells were transfected with three TET1-siRNAs (10 nM; 24 h) and (B) A2780s cells were transfected with TET1-cDNA (0.5 µg; 24 h), both followed by CDDP treatment (10 µM; 24 h). mRNA contents relative to ACTB (loading control) were assessed by qPCR. pGSN and GAPDH (loading control) protein contents were measured by Western blotting and apoptosis morphologically determined by Hoechst 33,258 nuclear staining (mean ± SEM; n = 3). p*<0.05, p**<0.01, p***<0.001, p****<0.0001