Fig. 2

Modulation of Gene Expression by Insulin in Decidualized hESCs. (A) This panel displays the relative mRNA expression of PRL, IGFBP1, BMP2, DUSP1, MMP2, and LEFTY2 in human endometrial stromal cells undergoing decidualization when exposed to insulin concentrations of 10, 50, and 200 nmol/L after a 4-day period. Data are shown as mean ± SD from three independent samples. Asterisks (*) indicate a significant difference (P < 0.05) when compared to the differentiated control group (De). (B-C) These panels show the influence of insulin on IGFBP1 protein expression, which is induced by cAMP over 2 days (B) and 4 days (C) of decidualization. The expression levels of IGFBP1 were quantitatively assessed through densitometric analysis using ImageJ software. Results are given as mean ± SD, based on a sample size of n = 3. Significance is noted by an asterisk (*) where P < 0.05