Fig. 4

The Role of NR4As in Regulating Decidualization of hESCs In Vitro. (A) Human endometrial stromal cells (hESCs) underwent transfection with 50 nmol/L of specific NR4A siRNAs for 24 h, followed by an additional 4 days of treatment with or without 0.5 mmol/L db-cAMP. Subsequent qRT-PCR assays measured the relative mRNA levels of decidual markers. Presented data represent mean ± SD for n = 3 samples, with * P < 0.05 indicating significance relative to the siCtrl plus db-cAMP group. (B-C) The transfection’s effectiveness for the NR4A1 gene and the corresponding IGFBP1 protein levels in hESCs were treated with 0.5 mmol/L db-cAMP and targeted siRNAs for decidualization periods of 2 (B) and 4 (C) days. Protein expression was quantitatively measured using ImageJ software. The results are shown as mean ± SD for n = 3 samples, with * P < 0.05 denoting significance when compared to siCtrl plus db-cAMP treatment