Fig. 3

Effect of trehalose on ovarian structure and number of follicles at all levels in mice. A HE stained smears of mouse ovaries in each group, a&d: Control group; b&e: PCOS group; c&f: trehalose group (a-c = 5 × microscope; d-f = 10 × microscope); B Statistical plot of the number of fifth-order ovarian follicles in each group of mice. N stands for control, P for PCOS, and S for trehalose. Data are presented as M + SD, N group compared with P group, P group compared with S group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001