Fig. 4

Effect of trehalose on oxidative stress and apoptosis in mouse ovarian mitochondria. A Ovarian Tunnel staining of mice in each group; B statistical diagram of ovarian Tunnel apoptosis rate in each group; C statistical diagram of total SOD content in the ovarian homogenate of mice in each group; D Protein bands of BAX, BCL-2, Cleaved caspase-9, SOD2, and GAPDH in ovaries of mice in each group. Each individual band is cropped and distinguished by a black box. The samples were from the same experiment and the gels were processed in parallel. E Statistical plots of relative amounts of BAX, BCL-2, Cleaved caspase-9, and SOD2 proteins in the ovaries of mice in each group, and the calculation equation was the gray value of target protein /GAPDH gray value. N stands for control, P for PCOS, and S for trehalose. Data are presented as M + SD, N group compared with P group, P group compared with S group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001