Fig. 5

Effect of trehalose on ovarian RAS expression in each group of mice. A Ovarian AGT mRNA expression in each group; B ovarian ACE mRNA expression; C ovarian AT1Ra mRNA expression in each group; D ovarian AT1Rb mRNA expression in each group; E ovarian AT2R mRNA expression in each group; F ovarian ACE2 mRNA expression in each group; G expression of ovarian MASR mRNA in each group; H ovarian AngII protein content in each group; I ovarian Ang1-7 protein content; J protein band diagram of ovarian RAS in each group. Each individual band is cropped and distinguished by a black box. The samples were from the same experiment and the gels were processed in parallel; K Statistical plots of relative protein expression of RAS in mouse ovaries of each group. N stands for control, P for PCOS, and S for trehalose. Data are presented as M + SD, N group compared with P group, P group compared with S group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001