Fig. 6

Effects of trehalose on intracellular oxidative stress and apoptosis and proliferation of KGN cells in each group. A ROS immunofluorescence staining of DCFH-DA probe in each group; B Statistical plot of differences in ROS levels of DCFH-DA probes in each group; C The fluorescence of JC-1 aggregate (red light), JC-1 monomer (green light), and combined fluorescence (red/green light); D Statistical plot of (JC-1 aggregate fluorescence intensity)/(JC-1 monomer fluorescence intensity) ratio in each group; E Hoechst(blue light), Azide 594(red light), and their combined light (red and blue light); F Numerical statistics of (Azide 594 red light spot number)/(Hoechst blue light spot number) in each group; G Protein bands of BAX, BCL-2, Cleaved caspase-9, SOD2, and GAPDH in KGN cells of each group; Each individual band is cropped and distinguished by a black box. The samples were from the same experiment and the gels were processed in parallel; H Statistical plots of relative amounts of BAX, BCL-2, Cleaved caspase-9, and SOD2 proteins in KGN cells of each group, were calculated by the gray value of target protein /GAPDH gray value. N stands for control, P for PCOS, and S for trehalose. Data are presented as M + SD, N group compared with P group, P group compared with S group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001