Fig. 7

Effect of trehalose on RAS expression in KGN cells of each group. A AGT mRNA expression in KGN cells of each group; B ACE mRNA expression in KGN cells; C AT1R mRNA expression in KGN cells of each group; D ovarian ACE2 mRNA expression in each group; E RAS protein band pattern of KGN cells in each group; Each individual band is cropped and distinguished by a black box. The samples were from the same experiment and the gels were processed in parallel; F Statistical plot of relative protein expression of RAS in KGN cells of each group. N stands for control, P for PCOS, and S for trehalose. Data are presented as M + SD, N group compared with P group, P group compared with S group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001