Fig. 4

EZTG can alleviate ferroptosis in the ovaries of aged mice. (A) Ovarian tissue sections with Prussian blue staining, scale bar + = 50 μm. (B) Observation of Mitochondrial morphology in ovaries under transmission electron microscopy, the yellow arrow identifies mitochondrial cristae, scale bar + = 1 μm. (C.D.E) Quantitative analysis of iron ions, GSH and MDA in ovaries. (F) Representative protein bands of GPX4 and ACSL4. (G.H) The corresponding quantitative statistical results of GPX4 and ACSL4 protein. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, All data are expressed as the mean ± SD and at least three independent experiments were performed