Fig. 1
From: Sestrin1, 2, and 3 are dispensable for female fertility in mice
Construction of Sesn1−/−, Sesn2−/−, and Sesn3−/− mice. (A) Limiting the expression of gene fragments for Sestrin1, Sestrin2, and Sestrin3 using the CRISPR/Cas9 system. (B) Genotyping of wild-type (WT) and knockout mice. (C) Detection of Sesn1, Sesn2 and Sesn3 mRNA in Sesn1−/−, Sesn2−/−, and Sesn3−/− and WT mice ovaries by Agarose gel electrophoresis of real time PCR. (D) Expression of SESN1, 2, and 3 in WT and knockout mice ovaries detected by IHC. Scale bar = 50 μm