Fig. 3

Comparison of ovarian reserve. (A) Representative micrographs of 12-month-old WT, Sesn1^−/−, Sesn2^−/−, and Sesn3^−/− mice ovarian sections. Scale bar = 500 μm. (B) Ovarian follicle count at developmental stages (primordial follicle (Pri), primary follicle (Prim), secondary follicle (Sec), and antral follicle (Ant) (n = 6 mice for each group). (C) Weights of ovaries in 3 M, 8 M, and 12 M WT, Sesn1^−/−, Sesn2^−/−, and Sesn3^−/− mice (n = 6 mice for each group). (D) Representative image of 12 months ovarian tissues with Masson’s trichrome staining. Scale bar = 500 μm. (E) The fibrosis area per ovarian section measured by ImageJ software. (F) The expression of p16, p21, and p53 in 12 M ovaries (n = 6 mice for each group). Gapdh was used as a loading control. Results are expressed as the mean ± SEM. Statistical differences between WT and knockout mice were measured by an unpaired Student’s t test