Fig. 4

Analysis of aging processes in ovaries. (A) Relative mRNA levels of Nlrp3, IL-1α, and TNF-α (n = 6 mice for each group). (B) Western blot for p62 protein in ovarian. GAPDH was used as a loading control (n = 6 mice for each group). (C) Representative images of TUNEL and γH2AX staining in 12 M WT, Sesn1^−/−, Sesn2^−/− and Sesn3^−/− mice. Scale bar = 100 μm. (D) Quantitative analysis of TUNEL and γH2AX positive cells (green) per 100 granulosa cells per antral follicle in WT, Sesn1^−/−, Sesn2^−/− and Sesn3^−/− mice in 12 months of age (n = 6 mice for each group). Results are expressed as the mean ± SEM. Statistical differences between WT and knockout mice were measured by an unpaired Student’s t test