Fig. 6
From: Loss of Calponin 2 causes premature ovarian insufficiency in mice
Calponin 2 expression in oocytes and cumulus cells. A Cumulus-oocyte-complexes (COCs) were collected from oviducts of a young WT female mouse 12 h after stimulated superovulation. After hyaluronidase digestion and removal of the cumulus cells, the COCs was stained with anti-calponin 2 mAb 1D11 and DAPI or Hoechst for nuclei. The representative images of a COC show that calponin 2 is expressed in both the oocyte and the surrounding cumulus cells. B Calponin 2 is detected homogenously in the ooplasm of the oocyte. C mAb 1D11 staining of a 4-cell embryo showed calponin 2 expression in the peripheral of the blastomeres. Bar = 40 μm