Fig. 7

Calponin 2 colocalizes with actin microfilaments, tropomyosin, and myosin II in ovarian granulosa cells. Granulosa cells were isolated from young female WT mice and examined with immunofluorescence microscopy after culturing for 48 h. A The representative images show that the granulosa cells are of polygonal epithelial-like morphology with flat and wide spreading cytoplasm (the arrow). B The arrowhead indicates a group of the granulosa cells displaying a cobble stone like appearance. C The cultured granulosa cells show significantly variable spreading areas of which some are much larger than others. The image of an example of large cells shows actin stress fibers in the cell body and on the edge of the cells and the colocalization of calponin 2 (the half arrowhead). D Colocalization of calponin 2 with tropomyosin (the arrowhead). E Colocalization of calponin 2 with myosin II (the arrowhead). F-actin was stained with rhodamine phalloidin. Nucleus counterstain was using DAPI