Table 1 Genes and primers used in the RT-qPCR
Gene | Primer | Sequence (5′ → 3′) |
|---|---|---|
TLR4 | Forward | CTGTATTCCCTCAGCACTCTTGATT |
Reverse | TGCTTCTGTTCCTTGACCCACT | |
MyD88 | Forward | CGCATGGTGGTGGTTGTT |
Reverse | CGCTTCTGTTGGACACCT | |
IKKα | Forward | GTCAGGACCGTGTTCTCAAGG |
Reverse | GCTTCTTTGATGTTACTGAGGGC | |
NF-κB | Forward | ACACTGGAAGCACGGATGAC |
Reverse | TGTCTGTGAGTTGCCGGTCT | |
TNF-α | Forward | CTCATGCACCACCATCAAGG |
Reverse | ACCTGACCACTCTCCCTTTG | |
IL-1β | Forward | TTCAGGCAGGCAGTATCACTC |
Reverse | GAAGGTCCACGGGAAAGACAC | |
IL-10 | Forward | CTTACTGACTGGCATGAGGATCA |
Reverse | GCAGCTCTAGGAGCATGTGG | |
IL-4 | Forward | CCATATCCACGGATGCGACA |
Reverse | AAGCACCTTGGAAGCCCTAC | |
Caspase-9 | Forward | AAGAAGACCGGAGTGCAATG |
Reverse | CATGACAGGATTATACAACCGC | |
Caspase-3 | Forward | GGAGGCTGACTTCCTGTATGCTT |
Reverse | CCTGTTAACGCGAGTGAGAATG | |
Bax | Forward | GATGGCAACTTCAACTGGG |
Reverse | CCGAAGTAGGAGAGGAGGC | |
Bcl-2 | Forward | CACTCGACCTTGTTTCTTCCAG |
Reverse | TCCTAACCCCTTGCTCTGCTT | |
β-actin | Forward | GCTCCTCCTGAGCGCAAGTA |
Reverse | CAGCTCAGTAACAGTCCGCC |