Fig. 4

BSHLD inhibited LPS-induced autophagy in GCs via reducing exosomal miR-30a-5p. Primary GCs were co-cultured with PBS or Exos derived from serum samples of sham, Model and BSHLD group rats. A The schematic diagram of the co-culture system of serum-derived Exos and GCs. B Expression of miR-30a-5p in GCs was measured by RT-qPCR. C Primary GCs were treated with miR‐30a-5p agomir or agomir NC for 48 h, and the expression of miR-30a-5p was assessed by RT-qPCR. D The viability of GCs was determined by CCK-8. E LC3B expression in GCs was evaluated by immunofluorescence staining. F The protein levels of ATG5, Beclin1, ULK1, LC3II/I, p-mTOR, mTOR, p-P70S6K, and P70S6K in GCs were evaluated by Western blotting. Results are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001