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Fig. 6 | Journal of Ovarian Research

Fig. 6

From: Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosis

Fig. 6

miR-30a-5p directly targeted SOCS3 to inactivate mTOR/P70S6K signaling. A The binding sites of miR-30a-5p on SOCS3 3’UTR were predicted by TargetScan database. B-C The direct binding of miR-30a-5p to SOCS3 was validated by dual luciferase reporter assay and RIP assay. D RT-qPCR analysis of SOCS3 level in GCs treated with miR‐30a-5p agomir or agomir NC. E The expression of SOCS3 was detected by RT-qPCR after transfection with lv-NC or lv- SOCS3. F Western blotting analysis of p-mTOR, mTOR, p-P70S6K, and P70S6K levels in GCs. Results are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001

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Journal of Ovarian Research

ISSN: 1757-2215

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