Fig. 7

Rapamycin reversed miR-30a-5p antagomir-mediated inhibitory role in GCs autophagy. A RT-qPCR analysis of miR‐30a-5p expression in GCs after treatment with miR‐30a-5p antagomir or NC antagomir. GCs were treated with miR-30a-5p antagomir alone or co-treated with miR-30a-5p antagomir + Rapamycin, and followed by exposure to LPS. B The viability of GCs was detected by CCK-8. C LC3B expression in GCs was observed by immunofluorescence staining. D The protein abundance of ATG5, Beclin1, ULK1, LC3II/I, p-mTOR, mTOR, p-P70S6K, and P70S6K in GCs was measured by Western blotting. Results are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001