Fig. 8

Rapamycin reversed miR-30a-5p antagomir-mediated inhibitory role on GCs pyroptosis. GCs were treated with miR-30a-5p antagomir alone or co-treated with miR-30a-5p antagomir + Rapamycin, and followed by exposure to LPS. A The release of IL-1β and IL-18 from GCs was evaluated by ELISA. B The percentage of pyroptotic GCs was detected by flow cytometry. C Western blotting measured the protein level of NLRP3. Results are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001