Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI medium)

Table 1 The composition of the control and FLI medium

Component	Supplier	Control medium	FLI medium
TCM199	Sigma, M7528	TCM199	TCM199
Sodium pyruvate	Sigma, P4562	0.2 mM	0.2 mM
L-glutamin	Sigma, G8540	6.85 mM	6.85 mM
Cysteine	Sigma, C7352	0.57 mM	0.57 mM
Gentamycin	Roth, 0233	50 µg/ml	50 µg/ml
BSA	Sigma, A7030	1 mg/ml	1 mg/ml
PMSG	Prospec¹, HOR-272	10 IU/ml	10 IU/ml
hCG	Prospec¹, HOR-250	10 IU/ml	10 IU/ml
EGF	PeproTech²,AF-100-15	10 ng/ml	10 ng/ml
human LIF	Merck, LIF1005	−	2 µl/ml
human IGF1	PeproTech, AF-100-11	−	20 ng/ml
human FGF2	Sigma, F0291	−	40 ng/ml

ISSN: 1757-2215