Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI medium)

Table 2 List of primers used for real-time RT-PCR

Gene trancript	Primers 5‘ –– 3‘	Amplicon length (bp)	T_an (°C)	GenBank accession number
AKT1	TAC TCC TTC CAG ACC CAC GA CGG AGT GCA GGT AGT CCA AG	157	53	NM_001159776.1
ATP6	AAT TCC TAT GCT CGT AAT ATG TTG AGT AGT GCT AAT	141	27	NC_000845
CCND2	CAG TGC TCC TAC TTC AAG ACC TCT TCT TCA CAC TTC	116	58	NM_214088.1
HAS2	GAA GTC ATG GGC AGG GAC AAT TC TGG CAG GCC CTT TCT ATG TTA	407	54	NM_214053.1
PCNA	TAA TGC AGA CAC CTT GGC ACT GCA AAT TCA CCA GAA GGC ATC	153	55	NM_001291925.1
TBP	ATA GCC TTC CAC CTT ACG CTC ATA GGC TGT GGA GTC AGT CCT	115	58	XM_021085483.1
YWHAG	CAG CCC ACT CAC CCC ATT AG TGC TGA TCG CTT GTC CAG AG	218	58	XM_005661962.3

ISSN: 1757-2215