Fig. 1

PCOS Data Acquisition and Identifying potential markers for HA PCOS specific genes. A Flowchart of the process of data procession progress, experimental design and analysis procedure for the microarray data. B Heatmap illustrating the 615 HA PCOS, NA PCOS and NM differentiated genes in GEO datasets and the microarray dataset with PCOS and some known PCOS and ovarian or breast tumor markers labeled on the right side. C The mRNA level measured by quantitative real-time PCR on the top 14 expressed genes on NA PCOS, HA PCOS and Normal (NM) groups for our microarray data. Data are expressed as means ± SEM. *p ≤ 0.05, Student t test. D Barplot showing the DAVID biological process analysis terms within the HA PCOS specific genes. Glucose metabolism terms and other terms were labeled red, and other terms were labeled as blue. E Barplot illustrating the HA PCOS specific genes on glucose metabolism terms(x-axis) and the corresponding number of glucose metabolism terms which each gene belonged (y-axis). F Co-occurrence results for CASR with PCOS, NA PCOS, HA PCOS and androgen receptor respectfully