Fig. 3

Experimental Validation of HA and NA PCOS Potential Marker in mRNA and protein level. A The mRNA expression of IL6R, CD274 and CASR in human granulosa cells from patients with HA PCOS (n = 4) and NA PCOS (n = 4) measured using quantitative real-time PCR. Data are expressed as means ± SEM. *p ≤ 0.05, Student t test. B and C The expression of IL6R and CD274 in human granulosa cells from HA PCOS (n = 4) and NA PCOS (n = 4) patients were subjected to Western blot analysis and values were normalized to GAPDH expression. Data are expressed as average and standard error of the mean (SE). The statistical analysis was performed using Student’s t test; *p ≤ 0.05 is considered significant