Fig. 5
From: Cai’s prescription inhibits granulosa cell apoptosis through ARHGAP4 on poor ovarian responders
ARHGAP4 regulates cell proliferation and apoptosis in vitro. (A) The expression level of ARHGAP4 was detected by western blot in KGN cells transfected with pCMV-tag 3A-ARHGAP4 or ARHGAP4 siRNA. (B) CCK-8 assay showed that ARHGAP4 overexpression significantly decreased the ability of KGN cell proliferation, and downregulation of ARHGAP4 showed inverse effect. Data are mean ± SD for three independent experiments. (C) Flow cytometry analysis of annexin-V and propidium iodide (PI) staining of apoptotic KGN cells with ARHGAP4 overexpression or downregulation