Fig. 6

The miR-1290/OGN axis in CAFs modulates tumorigenic capacity of ovarian cancer cell in vivo

Xenograft transplanted tumor models were established in nude mice by injecting a mixture of SKOV3 cells with CAFs or infected CAFs (infected with miR-1290 mimics or lv-OGN) and nude mice were separated into five groups (n = 6 per group): the SKOV3 cells + CAFs group, the SKOV3 cells + CAFs (lv-NC + NC mimics) group, the SKOV3 cells + CAFs (lv-OGN + NC mimics) group, the SKOV3 cells + CAFs (lv-NC + miR-1290 mimics) group, the SKOV3 cells + CAFs (lv-OGN + miR-1290 mimics) group. (A) Images of the tumors in each group. (B) Tumor volumes were measured every five days from the 10th day of the experiment. (C) Tumor weight were determined at the 30th day. (D–E) The mRNA levels of OGN and miR-1290 in mice tumor tissues were detected by qRT-PCR. (F) Ki67 and Vimentin levels in tumor tissues were examined using IHC staining. Scale bar = 20 μm. (G) The protein levels of E-cadherin, vimentin, p-mTOR, mTOR, p-Akt, and Akt in tumor tissues were determined using immunoblotting. *P < 0.05, **P < 0.01, compared between SKOV3 cells + CAFs group and SKOV3 cells + CAFs (lv-NC + NC mimics); ##P < 0.01 compared with SKOV3 cells + CAFs (lv-NC + NC mimics) group; &&P < 0.01 compared with SKOV3 cells + CAFs (lv-OGN + miR-1290 mimics) group