Fig. 2

The antitumor ability of mono-treatment and combination therapy in vitro. A Cytotoxicity of drugs on HM and OVCA429 cells was measured by CCK8. B PI (50 μg/mL) and Hoechst (10 μg/mL) staining was used to detect the dead cell after treatment. C The suppression effect of combination treatment on spheroid formation was evaluated in HM and OVCA429 cells. D The inhibitory effect of combination treatment on 3D spheroid formation was evaluated by Hoechst staining (10 μg/mL). Each data point represents the mean ± SEM of three independent experiments. ** p < 0.01, *** p < 0.001 and **** p < 0.0001 indicate statistical significance when compared to the control group