Fig. 3

Combination treatment aggravates ROS and 4-HNE accumulation and induces apoptosis. A lipid peroxides in HM and OCVA429 cells after individual or combination treatment was measured by BODIPY C11 staining and flow cytometry analysis. B confocal imaging (left) and quantification (right) of 4-HNE in HM and OVCA429 cells after individual and combination treatments. C Annexin V-FITC/PI staining and flow cytometry analysis was used to quantify cell apoptosis induced by drug treatment in HM and OVCA429 cells. The results were presented as stacked bars. D Flow cytometry was performed to assess the activity of caspase-3 in HM and OVCA429 cells after drug treatment for 48 h. Each experiment was performed in triplicate. Statistical significance was represented as *p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the control group